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Proteomic analysis of ACTN4-interacting proteins reveals it’s a putative involvement in mRNA metabolism

Mikhail Khotin, Lidia Turoverova, Vasilisa Aksenova, Nikolai Barlev, Veronika Viktorija Borutinskaite, Alexander Vener, Olga Bajenova, Karl-Eric Magnusson, George P. Pinaev, Dmitri Tentler

 

Biochemical and Biophysical Research Communications 397 (2010) 192–196

 

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ABSTRACT

Alpha-actinin 4 (ACTN4) is an actin-binding protein. In the cytoplasm, ACTN4 participates in structural organisation of the cytoskeleton via cross-linking of actin filaments. Nuclear localisation of ACTN4 has also been reported, but no clear role in the nucleus has been established. In this report, we describe the identification of proteins associated with ACTN4 in the nucleus. A combination of two-dimensional gel electrophoresis (2D-GE) and MALDI-TOF mass-spectrometry revealed a large number of ACTN4-bound proteins that are involved in various aspects of mRNA processing and transport. The association of ACTN4 with different ribonucleoproteins suggests that a major function of nuclear ACTN4 may be regulation of mRNA metabolism and signaling.

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