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Differential roles of p63 isoforms in epidermal development: selective genetic complementation in p63 null mice

E Candi, A Rufini, A Terrinoni, D Dinsdale, M Ranalli, A Paradisi, V De Laurenzi, LG Spagnoli, MV Catani, S Ramadan, RA Knight and G Melino


Cell Death and Differentiation (2006) 13, 1037–1047


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Epidermal development requires the transcription factor p63, as p63 mice are born dead, without skin. The gene expresses two proteins, one with an amino-terminal transactivation domain (TAp63) and one without (DNp63), although their relative contribution to epidermal development is unknown. To address this issue, we reintroduced TAp63a and/or DNp63a under the K5 promoter into p63 mice by in vivo genetic complementation. Whereas p63 and p63; TA mice showed extremely rare patches of poorly differentiated keratinocytes, p63;DN mice showed significant epidermal basal layer formation. Double TAp63a/ DNp63a complementation showed greater patches of differentiated skin; at the ultrastructural level, there was clear reformation of a distinct basal membrane and hemidesmosomes. At the molecular level, DNp63 regulated expression of genes characteristic of the basal layer (K14), interacting (by Chip, luc assay) with the third p53 consensus site. Conversely, TAp63 transcribed the upper layer’s genes (Ets- 1, K1, transglutaminases, involucrin). Therefore, the two p63 isoforms appear to play distinct cooperative roles in epidermal formation.


1. Candi E, Schmidt R and Melino G (2005) The cornified envelope: a model of cell death in the skin. Nat. Rev. Mol. Cell Biol. 6: 328–340.

2. Fuchs E and Watt FM (2003) Cell differentiation. Focus on epithelia. Curr. Opin. Cell Biol. 15: 738–739.

3. Owens DM and Watt FM (2003) Contribution of stem cells and differentiated cells to epidermal tumours. Nat. Rev. Cancer 3: 444–451.

4. Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Dotsch V, Andrews NC, Caput D and McKeon F (1998) p63, a p53 homolog at 3q27–29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol. Cell 2: 305–316.

5. Melino G, De Laurenzi V and Vousden KH (2002) p73: friend or foe in tumorigenesis. Nat. Rev. Cancer 2: 605–615.

6. Melino G, Lu X, Gasco M, Crook T and Knight RA (2003) Functional regulation of p73 and p63: development and cancer. Trends Biochem. Sci. 28: 663–670.

7. Yang A, Kaghad M, Caput D and McKeon F (2002) On the shoulders of giants: p63, p73 and the rise of p53. Trends Genet. 18: 90–95.

8. Yang A and McKeon F (2000) P63 and P73: P53 mimics, menaces and more. Nat. Rev. Mol. Cell Biol. 1: 199–207.

9. Celli J, Duijf P, Hamel BC, Bamshad M, Kramer B, Smits AP, Newbury-Ecob R, Hennekam RC, Van Buggenhout G, van Haeringen A, Woods CG, van Essen AJ, de Waal R, Vriend G, Haber DA, Yang A, McKeon F, Brunner HG and van Bokhoven H (1999) Heterozygous germline mutations in the p53 homolog p63 are the cause of EEC syndrome. Cell 99: 143–153.

10. Mills AA, Zheng B, Wang XJ, Vogel H, Roop DR and Bradley A (1999) p63 is a p53 homologue required for limb and epidermal morphogenesis. Nature 398: 708–713.

11. Yang A, Schweitzer R, Sun D, Kaghad M, Walker N, Bronson RT, Tabin C, Sharpe A, Caput D, Crum C and McKeon F (1999) p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 398: 714–718.

12. Bernassola F, Oberst A, Melino G and Pandolfi PP (2005) The promyelocytic leukaemia protein tumour suppressor functions as a transcriptional regulator of p63. Oncogene 24: 6982–6986.

13. Keyes WM, Wu Y, Vogel H, Guo X, Lowe SW and Mills AA (2005) p63 deficiency activates a program of cellular senescence and leads to accelerated aging. Genes Dev. 19: 1986–1999.

14. Lechler T and Fuchs E (2005) Asymmetric cell divisions promote stratification and differentiation of mammalian skin. Nature 437: 275–280.

15. Gressner O, Schilling T, Lorenz K, Schulze Schleithoff E, Koch A, Schulze- Bergkamen H, Maria Lena A, Candi E, Terrinoni A, Valeria Catani M, Oren M, Melino G, Krammer PH, Stremmel W and Muller M (2005) TAp63alpha induces apoptosis by activating signaling via death receptors and mitochondria. EMBO J. 24: 2458–2471.

16. Ghioni P, Bolognese F, Duijf PH, Van Bokhoven H, Mantovani R and Guerrini L (2002) Complex transcriptional effects of p63 isoforms: identification of novel activation and repression domains. Mol. Cell. Biol. 22: 8659–8668.

17. De Laurenzi V, Rossi A, Terrinoni A, Barcaroli D, Levrero M, Costanzo A, Knight RA, Guerrieri P and Melino G (2000) p63 and p73 transactivate differentiation gene promoters in human keratinocytes. Biochem. Biophys. Res.Commun. 273: 342–346.

18. Lapi E, Iovino A, Fontemaggi G, Soliera AR, Iacovelli S, Sacchi A, Rechavi G, Givol D, Blandino G and Strano S (2006) S100A2 gene is a direct transcriptional target of p53 homologues during keratinocyte differentiation. Oncogene [Epub ahead of print].

19. King KE, Ponnamperuma RM, Yamashita T, Tokino T, Lee LA, Young MF and Weinberg WC (2003) deltaNp63alpha functions as both a positive and a negative transcriptional regulator and blocks in vitro differentiation of murine keratinocytes. Oncogene 22: 3635–3644.

20. King KE, Ponnamperuma RM, Gerdes MJ, Tokino T, Yamashita T, Baker CC and Weinberg WC (2006) Unique domain functions of p63 isotypes that differentially regulate distinct aspects of epidermal homeostasis. Carcinogenesis 27: 53–63.

21. Ihrie RA, Marques MR, Nguyen BT, Horner JS, Papazoglu C, Bronson RT, Mills AA and Attardi LD (2005) Perp is a p63-regulated gene essential for epithelial integrity. Cell 120: 843–856.

22. Koster MI, Kim S, Mills AA, DeMayo FJ and Roop DR (2004) p63 is the molecular switch for initiation of an epithelial stratification program. Genes Dev. 18: 126–131.

23. McKeon F (2004) p63 and the epithelial stem cell: more than status quo? Genes Dev. 18: 465–469.

24. Nicotera P and Melino G (2005) Neurodevelopment on route p63. Neuron 48: 707–709. 25. Yuspa SH, Kilkenny AE, Steinert PM and Roop DR (1989) Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitro. J. Cell Biol. 109: 1207–1217.

26. Breuhahn K, Mann A, Muller G, Wilhelmi A, Schirmacher P, Enk A and Blessing M (2000) Epidermal overexpression of granulocyte–macrophage colonystimulating factor induces both keratinocyte proliferation and apoptosis. Cell Growth Differ. 11: 111–121.

27. Jessen H (1970) Two types of keratohyalin granules. J. Ultrastruct. Res. 33: 95–115. 28. Jacobs WB, Govoni G, Ho D, Atwal JK, Barnabe-Heider F, Keyes WM, Mills AA, Miller FD and Kaplan DR (2005) P63 is an essential proapoptotic protein during neural development. Neuron 48: 743–756.

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