Продажа новостроек от застройщика. Готовые новостройки продажа квартир от застройщика. Продажа новостроек в подмосковье. Продажа загородной недвижимости. Большой рынок загородной недвижимости. Обзор загородной недвижимости. Укладка линолеума на пол. Быстрая укладка линолеума своими руками. Цена укладка линолеума. Ремонт клавиатуры ноутбука. Быстрый ремонт клавиатуры своими руками. Сколько стоит ремонт клавиатуры. Потребительский кредит проценты. Кредит потребительский где взять. Потребительский кредит низкая процентная ставка. World of warcraft скачать торрент. Популярная игра world of warcraft. World of warcraft бесплатно. Журнал моделист конструктор. Журнал моделист конструктор онлайн. Моделист конструктор читать онлайн. Купить квартиру на первом этаже. Хороша ли квартира на первом этаже. Минусы квартиры на первом этаже.

Involvement of 5-lipoxygenase in programmed cell death of cancer cells

Mauro Maccarrone, Maria Valeria Catani, Alessandro Finazzi Agro and Gerry Melino


Cell Death and Differentiation (1997) 4, 396 - 402


Читать статью


We investigated the involvement of 5-lipoxygenase activity in the early phases of programmed cell death (PCD) induced by H2O2 or retinoids in different human tumour cells (erythroleukaemia, neuroblastoma, melanoma). Apoptotic cells showed enhanced 5-lipoxygenase activity which was paralleled by decreased superoxide dismutase activity and increased light emission. Ultraweak luminescence, mainly due to membrane lipid peroxidation by lipoxygenase activation, increased in all cell lines tested within 10 ± 15 min after induction of PCD, in a concentration and time-dependent manner. At the same time, we observed a significant increase in the intracellular steady state level of the 5-lipoxygenase metabolite leukotriene B4. Furthermore, 5-lipoxygenase metabolite 5-hydroxyeicosatetraenoic acidwas able toinduce PCD in all cell lines tested. Conversely, the general lipoxygenase inhibitor nordihydroguaiaretic acid and the selective 5-lipoxygenase inhibitor caffeic acid protected the different tumour cells from H2O2-induced PCD to a similar extent. These results show the activation of the 5- lipoxygenase pathway in PCD of three different cancer cell lines.


Abate C, Patel L, Rauscher III FJ and Curran T (1990) Redox regulation of Fos and Jun DNA-binding activity in vitro. Science 249: 1157 - 1161

Aebi H (1984) Catalase in vitro. Methods Enzymol 105: 121 - 126

Baker AF, BriehlMM,DorrRand PowisG(1996) Decreased antioxidant defense and increased oxidant stress during dexamethasone-induced apoptosis: bcl-2 selectively prevents the loss of catalase activity. Cell Death Differ 3: 87 - 93

Bissonnette RP, Brunner T, Lazarchik SB, Yoo NJ, Boehm MF, Green DR and Heyman RA (1995) 9-cis Retinoic acid inhibition of activation-induced apoptosis is mediated via regulation of Fas ligand and requires retinoic acid receptor and retinoid X receptor activation. Mol Cell Biol 15: 5576 - 5585

Boise LH, Gonzalez-Garcia M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, NunezGand ThompsonCB(1993) Bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 74: 597 - 608

Briehl MM, Cotgreave IA and Powis G (1995) Downregulation of the antioxidant defense during gluococorticoid-mediated apoptosis. Cell Death Differ 2: 41 - 46

Chittenden T, Harrington EA, O'Connor R, Flemington C, Lutz RJ, Evan GI and Guild BC(1995) Induction of apoptosis by the Bcl-2 homologue Bak. Nature 374: 733 - 736

De Laurenzi V, Melino G, Savini I, Annicchiarico-Petruzzelli M, Finazzi AgroÁ A and Avigliano L (1995) Cell death by oxidative stress and ascorbic acid regeneration in human neuroectodermal cell lines. Eur J Cancer 31A: 463 - 466

Devchand PR, Keller H, Peters JM, Vazquez M, Gonzalez, Whali W (1996) The PPARa-leukotriene B4 pathway to inflammation control. Nature 384: 39 - 43

Farrow SN, White JHM, Martinou I, Raven T, Pun KT, Grinham CJ, Martinou JC and BrownR(1995) Cloning of a bcl-2 homologue by interactionwith adenovirus E1B 19K. Nature 374: 731 - 733

Ford-Hutchinson AW, Gresser M and Young RN (1994) 5-Lipoxygenase. Annu Rev Biochem 63: 383 - 417

Hale AJ, Smith CA, Sutherland LC, Stoneman VEA, Longthorne VL, CulhaneACand Williams GT (1996) Apoptosis: molecular regulation of cell death. Eur J Biochem 236: 1 - 26

Hebert MJ, Takano T, Holthofer H, Bredy HR. (1996) Sequencial morphological events during apoptosis of human neutrophils. Modulation by lipoxygenasederived eicosanoids. J Immunol 157(7): 3105 - 3115

Hockenbery DM, Oltval ZN, Yin X-M, Milliman CL and Korsmeyer SJ (1993) Bcl- 2 functions in an antioxidant pathway to prevent apoptosis. Cell 75: 241 - 251

JacobsonMDandRaffMC(1995) Programmedcell deathandBcl-2 protection invery low oxygen. Nature 374: 814 - 816

Jakobsson P-J, Shaskin P, Larsson P, Feltenmark S, Odlander B, Aguilar-Santelises M, Jondal P and Claesson H-E (1995) Studies on the regulation and localization of 5-lipoxygenase in human B-lymphocytes. Eur J Biochem 232: 37 - 46

Kane DJ, Sarafian TA, Anton R, Hahan H, Butler Gralla EB, Valentine JS, Ord T and Bredesen DE (1993) Bcl-2 inhibition of neuronal death: decreased generation of reactive oxygen species. Science 262: 1274 - 1277

KleferMC,BrauerMJ,PowersVC,Wu JJ,UmanskySR,TomeiLDandBarrPJ (1995) Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. Nature 374: 736 - 739

KuÈhn H, Belkner J, Wiesner R and Brash AR (1990) Oxygenation of biological membranes by the pure reticulocyte lipoxygenase. J Biol Chem 265: 18351 -18361

Los M, Schenk H, Hexel K, Baeuerle PA, DrogeWand Schulze-Osthoff K (1995) IL-2 gene expression and NF-kB activation through CD28 requires reactive oxygen production by 5-lipoxygenase. EMBO J 14: 3731 - 3740

Maccarrone M, Bladergroen MR, Rosato N and Finazzi AgroÁ A (1995a) Role of lipid peroxidation in electroporation-induced cell permeability. Biochem Biophys Res Commun 209: 417 - 425

Maccarrone M, Rosato N and Finazzi AgroÁ A (1995b) Electroporation enhances cell membrane peroxidation and luminescence. Biochem Biophys Res Commun 206: 238 - 245

Martin SJ, TakayamaS,McGahon AJ, Miyashita T, Corbeil J, Kolesnick RN, Reed JC and Green DR (1995) Inhibition of ceramide-induced apoptosis by Bcl-2. Cell Death Differ 2: 253 - 257

McGahon AJ, Nishioka WK, Martin SJ, Mahboubi A, Cotter TGandGreen DR (1995) Regualtion of the Fas apoptotic cell death pathway by Abl. J Biol Chem 270: 22625 - 22631

MelinoG, Annicchiarico-Petruzzelli M, Piredda L, Candi E, Gentile V, DaviesPJAand Piacentini M (1994) Tissue-transglutaminase and apoptosis: sense and antisense transfection studies in human neuroblastoma cells. Mol Cell Biol 14: 6584 - 6596

Meyer M, Schreck R and Baeuerle PA (1993) H2O2 and antioxidants have opposite effects on activation of NF-kB and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor. EMBO J 12: 2005 - 2015

Nakano M, Ito T, Arimoto T, Ushijima Y and Kamiya K (1994) A simple luminescence method for detecting lipid peroxidation and antioxidant activity in vitro. Biochem Biophys Res Commun 202: 940 - 946

O'Donnell VB, Spycher S and Azzi A (1995) Involvement of oxidants and oxidantgenerating enzyme(s) in tumour-necrosis-factor-a-mediated apoptosis: role for lipoxygenase pathway but not mitochondrial respiratory chain. Biochem J 310: 133-141

Oltvai ZN, MillimanCL and Korsmeyer SJ (1993) Bcl-2 heterodimerizes in vivo with a concerved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619

Paoletti F and Mocali A (1990) Determination of superoxide dismutase acivity by a purely chemical system based on NAD(P)H oxidation. Methods Enzymol 186: 209-220

Piacentini M, Annicchiarico-Petruzzelli M, Oliverio S, Piredda L, Biedler JL and Melino G (1992) Phenotype-specific tissue transglutaminase regulation in human neuroblastoma cells in response to retinoic acid: correlation with cell death by apoptosis. Int J Cancer 52: 271 - 279

PiacentiniM, Fesus L and MelinoG(1993) Multiple cell cycle access to the apoptotic death programme in human neuroblastoma cells. FEBS Lett 320: 150 - 154

Raff M (1992) Social control on cell survival and cell death. Nature 356: 397 - 400

Sandstrom PA, Tebbey PW, Van Cleave S and Buttke TM (1994) Lipid hydroperoxides induce apoptosis in T cells displaying a HIV-associated glutathione peroxidase deficiency. J Biol Chem 269: 798 - 801

SchnurrK, HellwingM, Seidemann B, Jungblut P, KuÈhnH, RapoportSMand Schewe T (1996) Oxygenation of biomembranes by mammalian lipoxygenases: the role of ubiquinone. Free Rad Biol Med 20: 11 - 21

Shimizu S, Eguchi Y, Kosaka H, Kamiike W, Matsuda H and Tsujimoto Y (1995) Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL. Nature 374: 811 -813

SteinkuÈhlerC,Mavelli I,MelinoG,PiacentiniM, Rossi L,WeserUandRotilioG(1988) Antioxygenic enzyme activities in differentiating human neuroblastoma cells. Ann NY Acad Sci 551: 137 - 140

Steinman HM (1995) The Bcl-2 oncoprotein functions as a pro-oxidant. J Biol Chem 270: 3487 - 3490

Stoler DL, Anderson GR, Russo CA, Spina AM and Beerman TA (1992) Anoxiainducible endonuclease activity as a potential basis of the genomic instability of cancer cells. Cancer Res 52: 4372 - 4378

Sud'ina GF, Mirzoeva OK, Pushkareva MA, Korshunova GA, Sumbatyan NV and Varfolomeev SD (1993) Caffeic acid phenethyl ester as a lipoxygenase inhibitor with antioxidant properties. FEBS Lett 329: 21 - 24

Takayama S, Sato T, Krajewski S, Kochel K, Irie S, Millan JA and Reed JC (1995) Cloning and functional analysis of BAG-1: a novel Bcl-2-binding proteinwith anticell death activity. Cell 80: 279 - 284

Tang DG, Chen YQ and Honn KV (1996) Arachidonate lipoxygenases as essential regulators of cell survival and apoptosis. Proc Natl AcadSciUSA93:5241 - 5246

Torres-Roca JF, Lecoeur H, Amatore C and Gougeon ML (1995) The early intracellular production of reactive oxygen intermediate mediates apoptosis in dexamethasone-treated thymocytes. Cell Death Differ 2: 309 - 319

Van der Donk EMM, Verhagen J, Veldink GA and Vliegenthart JFG (1991) 12- Lipoxygenase from rat basophilic leukemia cells: separation from 5-lipoxygenase and temperature-dependent inactivation by hydroperoxy fatty acid. Biochim Biophys Acta 1081: 135 -140

Wolfe JT, Ross D and Cohen GM (1994) A role for metals and free radicals in the induction of apoptosis in thymocytes. FEBS Lett 352: 58 - 62

Yamamoto S (1992) Mammalian lipoxygenases: molecular structures and functions. Biochim Biophys Acta 1128: 117 - 131

Yang E, Zha J, Jockel J, Boise LH, Thompson GB and Korsmeyer SJ (1995) Bad, a heterodimeric partner for Bcl-Xl and Bcl-2, displaces Bax and promotes cell death. Cell 80: 285 - 291

Прочитано 1595 раз
Оцените материал
(0 голосов)
Опубликовано в СТАТЬИ
Авторизуйтесь, чтобы получить возможность оставлять комментарии


Управление научных исследований СПбГТИ (ТУ)

Горячие новости

Приборное оснащение лаборатории

Rambler's Top100
//'+ 'Рейтинг@Mail.ru<\/a><\/p>');})(window,navigator,document);//]]>