Снять квартиру посуточно. Быстро снять посуточно квартиру в казани. Снять однокомнатную квартиру посуточно. Помощь в получении ипотеки. Удобное получение ипотеки в сбербанке. Что нужно для получения ипотеки. Отопление частного дома. Как сделать отопление частный дом схема. Частный дом монтаж отопления. Восстановление жесткого диска. Быстрое восстановление информации с жесткого диска. Восстановление данных жесткого диска программа. Кредит с плохой историей. Быстрые кредиты онлайн с плохой историей. Срочный кредит с плохой историей. В какой сервер играть в WOW. Топ world of warcraft серверы. Wow серверы pandaria. Круглопалочный станок чертежи. Круглопалочный станок купить дешево. Самодельный круглопалочный станок. Кредит на покупку квартиры. На долго взять кредит на квартиру. Кредит на покупку квартиры.

miR-203 represses ‘stemness’ by repressing DNp63

AM Lena, R Shalom-Feuerstein, P Rivetti di Val Cervo, D Aberdam, RA Knight, G Melino and E Candi

 

Cell Death and Differentiation (2008) 15, 1187–1195

 

Читать статью


ABSTRACT

The epidermis, the outer layer of the skin composed of keratinocytes, is a stratified epithelium that functions as a barrier to protect the organism from dehydration and external insults. The epidermis develops depending on the transcription factor p63, a member of the p53 family of transcription factors. p63 is strongly expressed in the innermost basal layer where epithelial cells with high clonogenic and proliferative capacity reside. Deletion of p63 in mice results in a dramatic loss of all keratinocytes and loss of stratified epithelia, probably due to a premature proliferative rundown of the stem and transient amplifying cells. Here we report that microRNA (miR)-203 is induced in vitro in primary keratinocytes in parallel with differentiation. We found that miR-203 specifically targets human and mouse p63 30-UTRs and not SOCS-3, despite bioinformatics alignment between miR-203 and SOCS-3 30-UTR. We also show that miR-203 overexpression in proliferating keratinocytes is not sufficient to induce full epidermal differentiation in vitro. In addition, we demonstrate that miR-203 is downregulated during the epithelial commitment of embryonic stem cells, and that overexpression of miR-203 in rapidly proliferating human primary keratinocytes significantly reduces their clonogenic capacity. The results suggest that miR-203, by regulating the DNp63 expression level, is a key molecule controlling the p63-dependent proliferative potential of epithelial precursor cells both during keratinocyte differentiation and in epithelial development. In addition, we have shown that miR-203 can regulate DNp63 levels upon genotoxic damage in head and neck squamous cell carcinoma cells, thus controlling cell survival.


REFERENCES

1. Candi E, Schmidt R, Melino G. The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol 2005; 6: 328–340.

2. Blanpain C, Fuchs E. Epidermal stem cells of the skin. Ann Rev Cell Dev Biol 2006; 22: 339–373.

3. Senoo M, Pinto F, Crum CP, McKeon F. p63 Is essential for the proliferative potential of stem cells in stratified epithelia. Cell 2007; 129: 523–536.

4. Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Do¨ tsch V et al. p63, a p53 homolog at 3q27–29, encodes multiple products with transactivating, death-inducing, and dominantnegative activities. Mol Cell 1998; 2: 305–316.

5. Yang A, Schweitzer R, Sun D, Kaghad M, Walker N, Bronson RT et al. p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 1999; 398: 714–718.

6. Mills AA, Zheng B, Wang XJ, Vogel H, Roop DR, Bradley A. p63 is a p53 homologue required for limb and epidermal morphogenesis. Nature 1999; 398: 708–713.

7. Ambros V. The functions of animal microRNAs. Nature 2004; 431: 350–355.

8. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 16: 281–297.

9. Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol 2005; 6: 376–385.

10. Chen CZ, Li L, Lodish HF, Bartel DP. MicroRNAs modulate hematopoietic lineage differentiation. Science 2004; 303: 83–86.

11. Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE et al. A pancreatic islet-specific microRNA regulates insulin secretion. Nature 2004; 432: 226–230.

12. He L, Thomson JM, Hemann MT, Hernando-Monge E, Mu D, Goodson S et al. A microRNA polycistron as a potential human oncogene. Nature 2005; 435: 828–833.

13. O0Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT. c-Myc-regulated microRNAs modulate E2F1 expression. Nature 2005; 435: 839–843.

14. Zhao Y, Samal E, Srivastava D. Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis. Nature 2005; 436: 214–220.

15. Knight SW, Bass BL. A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans. Science 2001; 293: 2269–2271.

16. Bernstein E, Kim SY, Carmell MA, Murchison EP, Alcorn H, Li MZ et al. Dicer is essential for mouse development. Nat Genet 2003; 35: 215–217.

17. Murchison EP, Partridge JF, Tam OH, Cheloufi S, Hannon GJ. Characterization of Dicerdeficient murine embryonic stem cells. Proc Natl Acad Sci USA 2005; 102: 12135–12140.

18. Yi R, O0Carroll D, Pasolli HA, Zhang Z, Dietrich FS, Tarakhovsky A et al. Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs. Nat Genet 2006; 38: 356–362.

19. Andl T, Murchison EP, Liu F, Zhang Y, Yunta-Gonzalez M, Tobias JW et al. The miRNAprocessing enzyme dicer is essential for the morphogenesis and maintenance of hair follicles. Curr Biol 2006; 16: 1041–1049.

20. Sonkoly E, Wei T, Janson PC, Sa¨a¨ f A, Lundeberg L, Tengvall-Linder M et al. A MicroRNAs: novel regulators involved in the pathogenesis of Psoriasis? PLoS ONE 2007; 2: e610.

21. Rossi M, Aqeilan RI, Neale M, Candi E, Salomoni P, Knight RA et al. The E3 ubiquitin ligase Itch controls the protein stability of p63. Proc Natl Acad Sci USA 2006; 103: 12753–12758.

22. Candi E, Rufini A, Terrinoni A, Giamboi-Miraglia A, Lena AM, Mantovani R et al. DeltaNp63 regulates thymic development through enhanced expression of FgfR2 and Jag2. Proc Natl Acad Sci USA 2007; 104: 11999–12004.

23. Candi E, Rufini A, Terrinoni A, Dinsdale D, Ranalli M, Paradisi A et al. Differential roles of p63 isoforms in epidermal development: selective genetic complementation in p63 null mice. Cell Death Differ 2006; 13: 1037–1047.

24. Lee H, Kimelman D. A dominant-negative form of p63 is required for epidermal proliferation in zebrafish. Dev Cell 2002; 2: 607–616.

25. Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA. p63 regulates proliferation and differentiation of developmentally mature keratinocytes. Genes Dev 2006; 20: 3185–3197.

26. Barrandon Y, Green H. Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci USA 1987; 84: 2302–2306.

27. Gambaro K, Aberdam E, Virolle T, Aberdam D, Rouleau M. BMP-4 induces a Smaddependent apoptotic cell death of mouse embryonic stem cell-derived neural precursors. Cell Death Differ 2006; 13: 1075–1087.

28. Aberdam D, Gambaro K, Medawar A, Aberdam E, Rostagno P, de la Forest Divonne S et al. Embryonic stem cells as a cellular model for neuroectodermal commitment and skin formation. C R Biol 2007; 330: 479–484.

29. Rocco JW, Leong CO, Kuperwasser N, DeYoung MP, Ellisen LW. p63 mediates survival in squamous cell carcinoma by suppression of p73-dependent apoptosis. Cancer Cell 2006; 9: 45–56.

30. Tonon G, Wong KK, Maulik G, Brennan C, Feng B, Zhang Y et al. High-resolution genomic profiles of human lung cancer. Proc Natl Acad Sci USA 2005; 102: 9625–9630.

31. Hibi K, Trink B, Patturajan M, Westra WH, Caballero OL, Hill DE et al. AIS is an oncogene amplified in squamous cell carcinoma. Proc Natl Acad Sci USA 2000; 97: 5462–5467.

32. Forastiere A, Koch W, Trotti A, Sidransky D. Head and neck cancer. N Engl J Med 2001; 345: 1890–1900.

33. Rocco JW, Ellisen LW. p63 and p73: life and death in squamous cell carcinoma. Cell Cycle 2006; 5: 936–940.

34. Ratovitski E, Trink B, Sidransky D. p63 and p73: teammates or adversaries? Cancer Cell 2006; 9: 1–2.

35. DeYoung MP, Johannessen CM, Leong CO, Faquin W, Rocco JW, Ellisen LW. Tumorspecific p73 up-regulation mediates p63 dependence in squamous cell carcinoma. Cancer Res 2006; 66: 9362–9368.

36. Yi R, Poy MN, Stoffel M, Fuchs E. A skin microRNA promotes differentiation by repressing ‘stemness’. Nature 2008; 452: 225–229.

37. Zangen R, Ratovitski E, Sidransky D. DeltaNp63alpha levels correlate with clinical tumor response to cisplatin. Cell Cycle 2005; 4: 1313–1315.

38. Yuspa SH, Kilkenny AE, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitro. J Cell Biol 1989; 109: 1207–1217.

39. Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. Prediction of mammalian microRNA targets. Cell 2003; 115: 787–798.

Прочитано 3463 раз
Оцените материал
(0 голосов)
Опубликовано в СТАТЬИ
Авторизуйтесь, чтобы получить возможность оставлять комментарии

ПОДПИШИТЕСЬ НА НОВОСТИ

Управление научных исследований СПбГТИ (ТУ)

Горячие новости

Приборное оснащение лаборатории

Rambler's Top100
//'+ 'Рейтинг@Mail.ru<\/a><\/p>');})(window,navigator,document);//]]>