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New p63 targets in keratinocytes identified by a genome-wide approach

M Alessandra Vigano, Jerome Lamartine, Barbara Testoni, Daniele Merico, Daniela Alotto, Carlotta Castagnoli, Amelie Robert, Eleonora Candi, Gerry Melino, Xavier Gidrol and Roberto Mantovani

 

The EMBO Journal (2006) 25, 5105–5116

 

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ABSTRACT

p63 is a developmentally regulated transcription factor related to p53. It is involved in the development of ectodermal tissues, including limb, skin and in general, multilayered epithelia. The DNp63a isoform is thought to play a ‘master’ role in the asymmetric division of epithelial cells. It is also involved in the pathogenesis of several human diseases, phenotypically characterized by ectodermal dysplasia. Our understanding of transcriptional networks controlled by p63 is limited, owing to the low number of bona fide targets. To screen for new targets, we employed chromatin immunoprecipitation from keratinocytes (KCs) coupled to the microarray technology, using both CpG islands and promoter arrays. The former revealed 96 loci, the latter yielded 85 additional genes. We tested 40 of these targets in several functional assays, including: (i) in vivo binding by p63 in primary KCs; (ii) expression analysis in differentiating HaCaT cells and in cells overexpressing DNp63a; (iii) promoter transactivation and (iv) immunostaining in normal tissues, confirming their regulation by p63. We discovered several new specific targets whose functional categorization links p63 to cell growth and differentiation.


REFERENCES

Bamforth SD, Braganca J, Eloranta JJ, Murdoch JN, Marques FI, Kranc KR, Farza H, Henderson DJ, Hurst HC, Bhattacharya S (2001) Cardiac malformations, adrenal agenesis, neural crest defects and exencephaly in mice lacking Cited2, a new Tfap2 co-activator. Nat Genet 29: 469–474

Barbieri CE, Perez CA, Johnson KN, Ely KA, Billheimer D, Pietenpol JA (2005) IGFBP-3 is a direct target of transcriptional regulation by DeltaNp63alpha in squamous epithelium. Cancer Res 65: 2314–2320

Barbieri CE, Pietenpol JA (2006) p63 and epithelial biology. Exp Cell Res 312: 695–706

Barbieri CE, Tang LJ, Brown KA, Pietenpol JA (2006) Loss of p63 leads to increased cell migration and up-regulation of genes involved in invasion and metastasis. Cancer Res 66: 7589–7597

Beima KM, Miazgowicz MM, Lewis MD, Yan PS, Huang TH, Weinmann AS (2006) T-bet binding to newly identified target gene promoters is cell-type independent, but results in variable context-dependent functional effects. J Biol Chem 281:11992–12000

Beretta C, Chiarelli A, Testoni B, Mantovani R, Guerrini L (2005) Regulation of the cyclin-dependent kinase inhibitor p57Kip2 expression by p63. Cell cycle 4: 1625–1631

Bhattacharya R, Kang-Decker N, Hughes DA, Mukherjee P, Shah V, McNiven MA, Mukhopadhyay D (2005) Regulatory role of dynamin- 2 in VEGFR-2/KDR-mediated endothelial signaling. FASEB J 19: 1692–1694

Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM (1999) Functional role of p35srj, a novel p300/ CBP binding protein, during transactivation by HIF-1. Genes Dev 13: 64–75

Blais A, Dynlacht BD (2005) Devising transcriptional regulatory networks operating during the cell cycle and differentiation using ChIP-on-chip. Chromosome Res 13: 275–288

Braganca J, Eloranta JJ, Bamforth SD, Ibbitt JC, Hurst HC, Bhattacharya S (2003) Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. J Biol Chem 278: 16021–16029

Brooks CL, Gu W (2006) p53 ubiquitination: Mdm2 and beyond. Mol Cell 21: 307–315

Brunner HG, Hamel BC, Bokhoven Hv H (2002) P63 gene mutations and human developmental syndromes. Am J Med Genet 112: 284–290

Carroll DK, Carroll JS, Leong CO, Cheng F, Brown M, Mills AA, Brugge JS, Ellisen LW (2006) p63 regulates an adhesion programme and cell survival in epithelial cells. Nat Cell Biol 8: 551–561

Crosbie RH, Lim LE, Moore SA, Hirano M, Hays AP, Maybaum SW, Collin H, Dovico SA, Stolle CA, Fardeau M, Tome FM, Campbell KP (2000) Molecular and genetic characterization of sarcospan: insights into sarcoglycan–sarcospan interactions. Hum Mol Genet 9: 2019–2027

Dou J, Cui C, Dufour F, Alkon DL, Zhao WQ (2003) Gene expression of alpha-endosulfine in the rat brain: correlative changes with aging, learning and stress. J Neurochem 87: 1086–1100

Ellisen LW, Ramsayer KD, Johannessen CM, Yang A, Beppu H, Minda K, Oliner JD, McKeon F, Haber DA (2002) REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species. Mol Cell 10: 995–1005

Flores ER, Tsai KY, Crowley D, Sengupta S, Yang A, McKeon F, Jacks T (2002) p63 and p73 are required for p53-dependent apoptosis in response to DNA damage. Nature 416: 560–564

Fort P, Estrada FJ, Bordais A, Mornet D, Sahel JA, Picaud S, Vargas HR, Coral-Vazquez RM, Rendon A (2005) The sarcoglycan–sarcospan complex localization in mouse retina is independent from dystrophins. Neurosci Res 53: 25–33

Gaiddon C, Lokshin M, Ahn J, Zhang T, Prives C (2001) A subset of tumor-derived mutant forms of p53 down-regulate p63 and p73 through a direct interaction with the p53 core domain. Mol Cell Biol 21: 1874–1887

Ghioni P, Bolognese F, Duijf PH, Van Bokhoven H, Mantovani R, Guerrini L (2002) Complex transcriptional effects of p63 isoforms: identification of novel activation and repression domains. Mol Cell Biol 22: 8659–8668

Gressner O, Schilling T, Lorenz K, Schulze Schleithoff E, Koch A, Schulze-Bergkamen H, Maria Lena A, Candi E, Terrinoni A, Valeria Catani M, Oren M, Melino G, Krammer PH, Stremmel W, Muller M (2005) TAp63alpha induces apoptosis by activating signaling via death receptors and mitochondria. EMBO J 24: 2458–2471

Harms K, Nozell S, Chen X (2004) The common and distinct target genes of the p53 family transcription factors. Cell Mol Life Sci 61: 822–842

Heisler LE, Torti D, Boutros PC, Watson J, Chan C, Winegarden N, Takahashi M, Yau P, Huang TH, Farnham PJ, Jurisica I, Woodgett JR, Bremner R, Penn LZ, Der SD (2005) CpG Island microarray probe sequences derived from a physical library are representative of CpG Islands annotated on the human genome. Nucleic Acids Res 33: 2952–2961

Heron L, Virsolvy A, Peyrollier K, Gribble FM, Le Cam A, Ashcroft FM, Bataille D (1998) Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive KATP channel: molecular cloning, expression and biological properties. Proc Natl Acad Sci USA 95: 8387–8391

Hooper JE, Scott MP (2005) Communicating with Hedgehogs. Nat Rev Mol Cell Biol 6: 306–317

Ihrie RA, Marques MR, Nguyen BT, Horner JS, Papazoglu C, Bronson RT, Mills AA, Attardi LD (2005) Perp is a p63-regulated gene essential for epithelial integrity. Cell 120: 843–856

King KE, Ponnamperuma RM, Yamashita T, Tokino T, Lee LA, Young MF, Weinberg WC (2003) deltaNp63alpha functions as both a positive and a negative transcriptional regulator and blocks in vitro differentiation of murine keratinocytes. Oncogene 22: 3635–3644

Kirmizis A, Farnham PJ (2004) Genomic approaches that aid in the identification of transcription factor target genes. Exp Biol Med (Maywood) 229: 705–721

Koster MI, Kim S, Huang J, Williams T, Roop DR (2006) TAp63alpha induces AP-2gamma as an early event in epidermal morphogenesis. Dev Biol 289: 253–261

Koster MI, Kim S, Mills AA, DeMayo FJ, Roop DR (2004) p63 is the molecular switch for initiation of an epithelial stratification program. Genes Dev 18: 126–131

Koster MI, Roop DR (2004) The role of p63 in development and differentiation of the epidermis. J Dermatol Sci 34: 3–9

Kurata S, Okuyama T, Osada M, Watanabe T, Tomimori Y, Sato S, Iwai A, Tsuji T, Ikawa Y, Katoh I (2004) p51/p63 controls subunit alpha3 of the major epidermis integrin anchoring the stem cells to the niche. J Biol Chem 279: 50069–50077

Laurikkala J, Mikkola ML, James M, Tummers M, Mills AA, Thesleff I (2006) p63 regulates multiple signalling pathways required for ectodermal organogenesis and differentiation. Development 133: 1553–1563

Lebakken CS, Venzke DP, Hrstka RF, Consolino CM, Faulkner JA, Williamson RA, Campbell KP (2000) Sarcospan-deficient mice maintain normal muscle function. Mol Cell Biol 20: 1669–1677

Lee H, Kimelman D (2002) A dominant-negative form of p63 is required for epidermal proliferation in zebrafish. Dev Cell 2: 607–616

Lehman TA, Modali R, Boukamp P, Stanek J, Bennett WP,Welsh JA, Metcalf RA, Stampfer MR, Fusenig N, Rogan EM, Harris CC (1993) p53 mutations in human immortalized epithelial cell lines. Carcinogenesis 14: 833–839

Li D, Roberts R (2001) WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. Cell Mol Life Sci 58: 2085–2097

Liu G, Chen X (2002) The ferredoxin reductase gene is regulated by the p53 family and sensitizes cells to oxidative stress-induced apoptosis. Oncogene 21: 7195–7204

Maisse C, Guerrieri P, Melino G (2003) p73 and p63 protein stability: the way to regulate function? Biochem Pharmacol 66: 1555–1561

Mills AA, Zheng B, Wang XJ, Vogel H, Roop DR, Bradley A (1999) p63 is a p53 homologue required for limb and epidermal morphogenesis. Nature 398: 708–713

Nguyen BC, Lefort K, Mandinova A, Antonini D, Devgan V, Della Gatta G, Koster MI, Zhang Z,Wang J, di Vignano AT, Kitajewski J, Chiorino G, Roop DR, Missero C, Dotto GP (2006) Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation. Genes Dev 20: 1028–1042

Oberley MJ, Farnham PJ (2003) Probing chromatin immunoprecipitates with CpG-island microarrays to identify genomic sites occupied by DNA-binding proteins. Methods Enzymol 371: 577–596

Osada M, Park HL, Nagakawa Y, Begum S, Yamashita K, Wu G, Kim MS, Trink B, Sidransky D (2006) A novel response element confers p63- and p73-specific activation of the WNT4 promoter. Biochem Biophys Res Commun 339: 1120–1128

Osada M, Park HL, Nagakawa Y, Yamashita K, Fomenkov A, Kim MS, Wu G, Nomoto S, Trink B, Sidransky D (2005) Differential recognition of response elements determines target gene specificity for p53 and p63. Mol Cell Biol 25: 6077–6089

Pellegrini G, Dellambra E, Golisano O, Martinelli E, Fantozzi I, Bondanza S, Ponzin D, McKeon F, De Luca M (2001) p63 identifies keratinocyte stem cells. Proc Natl Acad Sci USA 98: 3156–3161

Praefcke GJ, McMahon HT (2004) The dynamin superfamily: universal membrane tubulation and fission molecules? Nat Rev Mol Cell Biol 5: 133–147

Rieger E, Bijl JJ, van Oostveen JW, Soyer HP, Oudejans CB, Jiwa NM, Walboomers JM, Meijer CJ (1994) Expression of the homeobox gene HOXC4 in keratinocytes of normal skin and epithelial skin tumors is correlated with differentiation. J Invest Dermatol 103: 341–346

Sasaki Y, Ishida S, Morimoto I, Yamashita T, Kojima T, Kihara C, Tanaka T, Imai K, Nakamura Y, Tokino T (2002) The p53 family member genes are involved in the Notch signal pathway. J Biol Chem 277: 719–724

Sasaki Y, Naishiro Y, Oshima Y, Imai K, Nakamura Y, Tokino T (2005) Identification of pigment epithelium-derived factor as a direct target of the p53 family member genes. Oncogene 24: 5131–5136

Senoo M, Matsumura Y, Habu S (2002) TAp63gamma (p51A) and dNp63alpha (p73L), two major isoforms of the p63 gene, exert opposite effects on the vascular endothelial growth factor (VEGF) gene expression. Oncogene 21: 2455–2465

Stelnicki EJ, Komuves LG, Kwong AO, Holmes D, Klein P, Rozenfeld S, Lawrence HJ, Adzick NS, Harrison M, Largman C (1998) HOX homeobox genes exhibit spatial and temporal changes in expression during human skin development. J Invest Dermatol 110: 110–115

Svard J, Henricson KH, Persson-Lek M, Rozell B, Lauth M, Bergstrom A, Ericson J, Toftgard R, Teglund S (2006) Genetic elimination of suppressor of fused reveals an essential repressor function in the mammalian hedgehog signaling pathway. Dev Cell 10: 187–197

Testa A, Donati G, Yan P, Romani F, Huang TH, Vigano MA, Mantovani R (2005) Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters. J Biol Chem 280: 13606–13615

Vihanto MM, Plock J, Erni D, Frey BM, Frey FJ, Huynh-Do U (2005) Hypoxia up-regulates expression of Eph receptors and ephrins in mouse skin. FASEB J 19: 1689–1691

Wang X, Bolotin D, Chu DH, Polak L, Williams T, Fuchs E (2006) AP-2alpha: a regulator of EGF receptor signaling and proliferation in skin epidermis. J Cell Biol 172: 409–421

Westfall MD, Mays DJ, Sniezek JC, Pietenpol JA (2003) The Delta Np63 alpha phosphoprotein binds the p21 and 14-3-3 sigma promoters in vivo and has transcriptional repressor activity that is reduced by Hay-Wells syndrome-derived mutations. Mol Cell Biol 23: 2264–2276

Wu G, Nomoto S, Hoque MO, Dracheva T, Osada M, Lee CC, Dong SM, Guo Z, Benoit N, Cohen Y, Rechthand P, Califano J, Moon CS, Ratovitski E, Jen J, Sidransky D, Trink B (2003) DeltaNp63alpha and TAp63alpha regulate transcription of genes with distinct
biological functions in cancer and development. Cancer Res 63: 2351–2357

Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Dotsch V, Andrews NC, Caput D, McKeon F (1998) p63, a p53 homolog at 3q27–29, encodes multiple products with transactivating, deathinducing, and dominant-negative activities. Mol Cell 2: 305–316

Yang A, Schweitzer R, Sun D, Kaghad M, Walker N, Bronson RT, Tabin C, Sharpe A, Caput D, Crum C, McKeon F (1999) p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 398: 714–718

Yee J, Cortes P, Barnes JL, Alviani R, Biederman JI, Szamosfalvi B (2004) Rat mesangial alpha-endosulfine. Kidney Int 65: 1731–1739

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